Application Note - Mutation detection in circulating tumor cell samples obtained from the IsoFlux™ System
Here we present a novel KRAS mutation detection assay that can be run on CTCs obtained from the IsoFlux System (Fluxion Biosciences, South San Francisco, CA). The IsoFlux System is a benchtop instrument and microfluidic chip that efficiently recovers rare CTCs from blood samples and passes them off for further testing.
For the downstream KRAS mutational assay, a sensitive allele-specific Taqman based qPCR approach was utilized (castPCR, Life Technologies). The combination of these two technologies provides a complete, ‘sample-to-answer’ workflow for mutation detection.