Temperature Dependence of hERG Blocker Pharmacology - An Automated Patch Clamp Study

Abstract:

The hERG ion channel (Kv11.1) is at the center of preclinical cardiac safety assessment. Historically, patch clamp measure- ments that are used to assess hERG block of candidate com- pounds have been done at room temperature. While record- ing at physiological temperatures is challenging in terms of es- tablishing and maintaining a high sealing resistance, record- ings at elevated temperature are feasible using recently intro- duced automated patch clamp systems are capable of controlling temperature.

In this study we compare hERG current voltage relations and kinetics at different temperatures, and report observed chang- es in the pharmacology of known blockers using a next gen- eration automated patch clamp platform (IonFlux).

(Presented at Biophysics 2011) 

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