IonFlux: Enabling “Plate Reader” Simplicity in High Throughput Electrophysiology through Microfluidic Integration

ABSTARCT

While automated electrophysiology systems have been developed to record from individual cells and groups of cells, both approaches have limitations. Platforms that record from individual cells suffer from relatively low recording success rates, which typically hover around 50%. Coupled with the need to get a sufficient number of data points for statistically relevant results, the resulting experimental throughput is compromised. Systems that record from groups of cells provide an inherent averaging mechanism that increases recording success.

The IonFlux system is the first platfrom to market that combines ensem le recording with continuous voltage clamp and fast compound exchange. The system integrates microfluidic flow channels and cell trapping sites into SBS-standard 384-well plates, allowing for cell/compound dispensing using standard liquid handlers. Because each compound channel is individually controlled via pneumatic pressure, the plate-reader like instrument can apply any compound to any number of cell groups simultaneously, with an ‘on’ time of 100ms.

 

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