This document is intended to guide the user through a method for using multiple antibody types conjugated to magnetic beads to recover circulating tumor cells (CTCs). This protocol provides specific instructions for the use of EpCAM, EGFR, and HER-2 antibodies, and guidelines for protocol optimization if other surface markers are to be used. The protocol incorporates use of the IsoFlux System and the IsoFlux Rare Cell Enrichment Kit.
This protocol has been validated by Fluxion using tumor cell line spike-in experiments.
Please consult Fluxion for any updates to this protocol.