Shear flow conditions enabled by the BioFlux system (Figure 1) can be used to either stimulate physiological conditions (e.g. urinary tract, gastrointestinal tract, respiratory tract, blood stream) or used for introduction of organisms and buffers during uninterrupted microscopic observation. In vitro models can be developed for the study of host pathogen interactions that utilize both benefits of this system.
Cystic fibrosis (CF), a genetic disorder affecting cellular ion transport, is complicated by chronic lung infections by a variety of opportunistic bacteria, including P. aeruginosa. Study of host-pathogen interactions in CF is germane to discovering how the infections are established and how to prevent them. Here we present a model of airway epithelial cells and P. aeruginosa in the BioFlux system.