An improved medium for growing Staphylococcus aureus biofilm.

J Microbiol Methods. 2012 Aug;90(2):115-8. doi: 10.1016/j.mimet.2012.04.009. Epub 2012 Apr 19.

An improved medium for growing Staphylococcus aureus biofilm.

Chen PAbercrombie JJJeffrey NRLeung KP.


Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, TX 78234, USA.


A medium (Brain Heart Infusion plus 10% human plasma) was developed, tested, and validated for growing Staphylococcus aureus biofilm in vitro. With this medium, S. aureus forms reproducible and robust biofilms in flow chambers under controlled shear flow and with increased viability recovery in static well plates.

Copyright © 2012. Published by Elsevier B.V.


Keywords: Microbiology