Samarian, D. S., Jakubovics, N. S., Luo, T. L., Rickard, A. H.
J. Vis. Exp. (94), e52467, doi:10.3791/52467 (2014).
The goal of this methods paper is to describe the use of a microfluidic system for the development of multi-species biofilms that contain species typically identified in human supragingival dental plaque. Methods to describe biofilm architecture, biofilm viability, and an approach to harvest biofilm for culture-dependent or culture-independent analyses are highlighted.
Keywords: Microbiology, Biofilm