Assay Reproducibility: Assessing experimental variance in an adhesion assay using BioFlux Plates

BioFlux 200 Plate microfluidic channels were coated with human fibronectin (10 μg/ml) for 1 hour. Channels were washed with RPMI +10mM hepes for 10 minutes (37°C). Cells were added to the inlet A wells, perfused for 5 minutes at 2 dyn/cm2, and washed with PBS from inlet B for 10 minutes at 10 dyn/cm2. Images were captured at random beginning at the outlet end of the viewing window. 

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