Endothelial Cell Preconditioning: Incubation at 5% CO2 versus pre-conditioning in CO2 -independent media

For certain studies involving cells in the BioFlux 200 system, it is desirable to keep cells in close proximity to the microscope while growing or preconditioning them for up to 24 hours. Because traditional cell culture growth media requires CO2 to maintain pH, keeping cells in ambient gas in normal media will cause an undesirable effect on pH leading to deleterious effects on the monolayers of interest. Additionally, it is not always feasible to have a tissue culture incubator in a microscope facility. For these reasons, we compared cells preconditioned in CO2-independent media (CO-ind) (Invitrogen) on the BioFlux heater plate and in CO2 -dependent media in the incubator.