Cellular Monolayers: Growing airway epithelial cells in the BioFlux microfluidic channels

Cystic fibrosis (CF) is an inherited disease which affects the lungs and digestive systems. In CF, the airway epithelium fails to initiate an appropriate innate immune response which is needed for bacterial clearance. In addition, the opportunistic pathogen Pseudomonas Aeruginosa (PA) is difficult to eradicate due to its propensity to form biofilms. To model the interactions of PA and epithelium, a monolayer of airway epithelial cells as the adhesion substrate is required to study the infection of the opportunistic pathogen with epithelium.