Platelet Adhesion and Aggregation: Assay protocol for physiological shear ranges

The BioFlux system offers a physiologically-relevant platform for running vascular biology assays such as platelet adhesion and thrombosis. These assays often require the use of whole blood or its components to maximize biological relevance. Fluxion’s Well Plate Microfluidic technology makes it easy to run whole blood assays because all of the fluidic reagents are neatly contained in the well plate rather than being distributed through a messy network of tubing. The microfluidic format uses substantially less blood (up to 100 times less) than conventional flow cells. This enables more experimental conditions using the same human donor or mouse model.

This protocol is for running platelet adhesion and aggregation assays on various extracellular matrix proteins at physiological shear flow rates.