Cells and reagents can be prepared using standard lab techniques. It is important to adjust the cell density to an amount appropriate for a microfluidic flow channel. Common values range from 2x106- 1x107 cells/ml for mammalian cells. This is a general guideline and should be used as a starting point.
When adding reagents, care should be taken to place the tip of the pipette in close proximity to the bottom of the central lower well of the plate. Doing so reduces the chance of air bubbles being caught in the channels.