Tutorial: BioFlux Montage, Multi‐Wavelength Cell Scoring

This tutorial will show how to use the MultiWavelength Cell Scoring Module in the BioFlux Montage software. This module automatically detects the presence of up to 7 fluorescent stains within each cell. The module identifies a variety of useful measurements including the number and percent of cells scored positive for each marker, as well as individual cell scoring profiles across the set of markers. In this example, we will look at stem cells stained with a nuclear and cytoplasm stain.