Working with Plate Sealing Film

If you see a gap along the perimeter after you place the film on the plate, lab tape placed along the outside edge (long side) of the plate over the film will seal it better than film alone.

When you punch the plate with a needle, try to center the punch inside the well. You only need to punch the wells that you are working with.

It is easier to peel the film back than to punch through it for liquid changes.