Method Optimization for the Measurement of Exposed CD62P on Platelet Surface


P-selectin (CD62P) exposure on platelets has
been used as an indicator of platelet activation
as it is translocated from alpha granules
to the plasma membrane during platelet
secretion. The most commonly used method
to detect CD62P exposure on platelets is by
flow cytometry. A literature survey indicated
that a broad range of CD62P levels have been
reported for the same type of platelet product
(Table 1). Methodological differences in
measurement of platelet surface CD62P most
likely account for such wide spread values.