Spotlight 59 Oncology Panel- Data Sheet

Designed specifically for detection of mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for Illumina sequencers, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight 59 makes it ideal for CTCs, cfDNA, and other challenging sample types such as FFPE. Combined with ERASE-Seq, Fluxion’s statistically powered variant caller, Spotlight 59 delivers 100% specificity at 0.2% allele frequency (no false positives across 27.5kb panel). Used in conjunction with Fluxion’s IsoFlux Liquid Biopsy System, Spotlight 59 creates a complete sample-to-answer assay for high sensitivity mutation detection of solid tumor cancers from blood samples.

Key Features:

  • 59 oncology-related genes, 277 amplicons
  • Detection to 0.1% allele frequency
  • No false positives across 27.5kb panel (average specificity >99.999%)
  • Turnkey solution for library generation in a single kit, including sequencing adapters
  • Ready-to-sequence libraries in just 2 hours
  • Starting input quantities as low as 200 cells, or 20 ng DNA
  • Average amplicon size of 140 bp for compatibility with cfDNA and FFPE
  • ERASE-Seq variant calling included with kit purchase
  • No MID (molecular IDs) required; increases sensitivity and reduces bias
  • Vaidated on Illumina MiniSeq, MiSeq, and HiSeq
  • No primer trimming required