BioFlux Plate Channel Dimensions

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BioFlux plates have microfluidic channels that connect adjacent wells, forming a microfluidic flow cell. The plates come in a range of configurations to meet different cell biology applications. Channel layout and geometry has been optimized for each format. 

The table below summarizes the dimensions for the various plate configurations. 






910-0050 910-0110
48-well low shear 48-well high shear 24-well low shear 6-well plate
Channel height (all channels, microns) 70 75 75 100 (50 for viewing channel)
Channel length, inlet to viewing region (mm) 210 7.3 90 411.5
Channel width, inlet to viewing region (microns) 90 430 60 100
Channel length, viewing region (mm) 4 8 8 7.5
Channel width, viewing region (microns) 350 250 350 370
Channel length, viewing to outlet (mm) 9.6 46.3 28.9 34.5
Channel width, viewing to outlet (microns) 350 150 270 200


The illustration below shows an example of a viewing channel in a typical BioFlux plate. The viewing channel is wider than it is tall to allow for a wide zone of constant shear within the channel, when imaged from below using an inverted microscope.

Frequently asked questions

Why is there a long region of "serpentine" channel in each design?

This channel adds fluidic resistance and improves the control and accuracy of flow rate and shear stress. See the BioFlux plates page for channel layout details. 

Why is the serpentine section after the viewing channel in the high shear plates, while it is located before the viewing channel in the low shear plates?

High shear plates are typically used for thrombus studies. Keeping the channel large upstream of the viewing window keeps the shear stress low in that region, so the platelets aren't activated upstream. Having the high-shear region start in the viewing window allows users to see and image the thrombus as it is forming in the viewing window.

Why is the height of the viewing channel different in the 6-well plate?

The 6-well plate is designed for long-term cell culture experiments, which can often last days. The lower height of the 6-well plate viewing channel (50 microns) allows the desired shear stress to be generated at a lower flow rate, extending run time.